Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited
Assay Code APB-003
Description Bluetongue (BT) is an infectious, non -contagious, vector-borne viral disease that affects wild and domestic ruminants such as sheep, goats, cattle, buffaloes, deer, most species of African antelope and various other vertebrate hosts. It is transmitted by midges, small insects, of the genus Culicoides. In sheep,Bluetongue virus (BTV) causes an acute disease with high morbidity and mortality. Infection of cattle with BTV does not usually result in clinical signs, with the exception of BTV 8 infection in Europe. Cattle are particularly significant in the epidemiology of the disease due to the prolonged viraemia in the absence of clinical disease. Clinical signs of BT include fever, hyperaemia and congestion, swelling of the face and tongue and cyanosis of the tongue. However in mild cases of the disease, a transitory hyperaemia and slight ocular and nasal discharge may be observed.

Method Real-Time RT-PCR
Sample Type
Accredited : EDTA Blood.
Alternatives : Culture, Tissue.
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code APB-193
Description Bornavirus is a non-enveloped negative strand ribonucleic acid (RNA) virus. Because it uniquely replicates in the cell nucleus, it has been classified in its own family, Bornaviridae. In 2008, ABV was identified as the causative agent of proventricular dilatation disease (PDD) in psittacines, but the primary reservoir for ABV appears to be waterfowl. 

The epidemiology of avian bornavirus infections is poorly characterized, including the natural reservoir(s) of infection and routes of virus transmission. The incubation period in experimentally infected birds ranges from 20 to 200 days after inoculation. Bornavirus RNA can be detected in the feces and cloacal and crop contents of naturally infected birds

Method Real -Time RT-PCR.
Sample Type
EDTA Blood, Tissue (Brain), Swab/ Secretion (Respiratory), Swab/ Secretion (Rectal)
Transport Condition Samples should be transported at 4°C. Tissue and swabs must be sent in RNA preservative media.
It is not required to add RNA preservative media to blood.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APB-004
Description Bovine virus diarrhea or bovine viral diarrhea (BVD)or bovine viral diarrhoea (UK English) is a disease of cattle which reduces productivity and increases death loss. It is caused by a Pestivirus from the family Flaviviridae. Classical swine fever (CSF) is also caused by a pestivirus. CSF and sometimes BVD are notifiable diseases and eradication programs are administered in many countries worldwide. The molecular biology of pestiviruses shares many similarities and peculiarities with the human hepaciviruses. Pestiviruses have the ability to establish persistent infection during pregnancy. Persistent infection with pestiviruses often goes unnoticed; for BVDV frequently nonhomologous RNA recombination events lead to the appearance of genetically distinct viruses that are lethal to the host.

Method Real time RT-PCR
Sample Type
Accredited : Tissue (lung, spleen), Culture, Milk
Alternatives : EDTA Blood, Semen, Swab/Secretion (Respiratory), Swab/Secretion (Rectal)
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited
Assay Code APB-003
Description Bluetongue (BT) is an infectious, non -contagious, vector-borne viral disease that affects wild and domestic ruminants such as sheep, goats, cattle, buffaloes, deer, most species of African antelope and various other vertebrate hosts. It is transmitted by midges, small insects, of the genus Culicoides. In sheep,Bluetongue virus (BTV) causes an acute disease with high morbidity and mortality. Infection of cattle with BTV does not usually result in clinical signs, with the exception of BTV 8 infection in Europe. Cattle are particularly significant in the epidemiology of the disease due to the prolonged viraemia in the absence of clinical disease. Clinical signs of BT include fever, hyperaemia and congestion, swelling of the face and tongue and cyanosis of the tongue. However in mild cases of the disease, a transitory hyperaemia and slight ocular and nasal discharge may be observed.

Method Real-Time RT-PCR
Sample Type
Accredited : EDTA Blood.
Alternatives : Culture, Tissue.
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code APB-193
Description Bornavirus is a non-enveloped negative strand ribonucleic acid (RNA) virus. Because it uniquely replicates in the cell nucleus, it has been classified in its own family, Bornaviridae. In 2008, ABV was identified as the causative agent of proventricular dilatation disease (PDD) in psittacines, but the primary reservoir for ABV appears to be waterfowl. 

The epidemiology of avian bornavirus infections is poorly characterized, including the natural reservoir(s) of infection and routes of virus transmission. The incubation period in experimentally infected birds ranges from 20 to 200 days after inoculation. Bornavirus RNA can be detected in the feces and cloacal and crop contents of naturally infected birds

Method Real -Time RT-PCR.
Sample Type
EDTA Blood, Tissue (Brain), Swab/ Secretion (Respiratory), Swab/ Secretion (Rectal)
Transport Condition Samples should be transported at 4°C. Tissue and swabs must be sent in RNA preservative media.
It is not required to add RNA preservative media to blood.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APB-004
Description Bovine virus diarrhea or bovine viral diarrhea (BVD)or bovine viral diarrhoea (UK English) is a disease of cattle which reduces productivity and increases death loss. It is caused by a Pestivirus from the family Flaviviridae. Classical swine fever (CSF) is also caused by a pestivirus. CSF and sometimes BVD are notifiable diseases and eradication programs are administered in many countries worldwide. The molecular biology of pestiviruses shares many similarities and peculiarities with the human hepaciviruses. Pestiviruses have the ability to establish persistent infection during pregnancy. Persistent infection with pestiviruses often goes unnoticed; for BVDV frequently nonhomologous RNA recombination events lead to the appearance of genetically distinct viruses that are lethal to the host.

Method Real time RT-PCR
Sample Type
Accredited : Tissue (lung, spleen), Culture, Milk
Alternatives : EDTA Blood, Semen, Swab/Secretion (Respiratory), Swab/Secretion (Rectal)
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links